Research Article
High efficiency direct in vitro regeneration from different explants of medicinal plant turkey berry (solanum torvum sw)
Author(s): Ghan Singh Malothu, Rajinikanth Marka and Rama Swamy Nanna*
In the present investigation the role of different plant growth regulators (PGRs) on direct regeneration from various explants, cotyledon, hypocotyl and leaf was studied to find out the regeneration potentiality of the explant and also role of PGRs in Turkey berry/pea egg plant, S. torvum. The explants such as hypocotyl (0.4-0.8 cm long), cotyledon (0.6-0.8 cm2) from 3 week old and leaves (1.0 cm2) from 6 week old in vitro grown seedlings were excised and cultured on MS medium with various concentrations of BAP/KIN alone and also in combination with 0.5-1.0 mg/L IAA showed the direct shoots regeneration. All the explants have shown the maximum percentage of response (>90%) and highest number of direct induction of multiple shoots per explant (55±0.15 cotyledon, 52±0.13 leaf and 31±0.17 hypocotyl explants) was found at 0.5 mg/L IAA+2.5 mg/L BAP in comparison to .. View More»
DOI:
http://dx.doi.org/10.14303/irjps.2021.31