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Clinical Microbiology 2015: Genotype I and IV of dengue virus DEN-1 and its clinical manifestasions in Surabaya- Puspa Wardhani- Universitas Airlangga
Author(s): Puspa Wardhani
Previous studies showed there has been a Dengue Virus (DENV) distribution shift from DENV-2 to DENV-1 in Surabaya. This study analysed genetic characteristics of DENV-1 and clinical manifestations. This was an analytical observational study with prospective cohort setting. The study was conducted in Dr. Soetomo General Hospital, from February to August 2012. DENV serotyping was done by real-time PCR using SimplexaTM 3M RT-PCR instrument. Viral load examination was done by two step real-time PCR in Applied Bio system 7500 instrument. Positive samples were cultured in C6/36 cells. Positive culture samples were genotyped using envelope gene sequence. Dengue serotype d.. View More»