Short Communication
Modification of the goat mammary gland glycosylation pathway by overexpression of GnT-IVa
Author(s): Maria Jose Leiva Carrasco*
Statement of the Problem: Recombinant glycoprotein
expression has been carried out in different expression
systems based on the genetic modification. Specifically,
recombinant glycoproteins whose biological activity
depends on post-translational modifications are produced
in cell tissue culture, which increases the manufacturing
cost. The production of biotherapeutics in the mammary
glands of genetically modified mammals results in an
alternative method to overcome the drawback of cell
culture expression system. However, the N-glycosylation
pattern of complex glycoproteins produced in the
mammary epithelia has showed diminished antennae
formation and lower sialic acid contents compare to
native protein. An alternative to obtain high- quality
biopharmaceuticals in milk could be the modification of
the N-glycosylation pattern by overexpression of
exogenous glycosyltransfera.. View More»