International Research Journal of Biotechnology

Abstract

As a nanofiber hydrogel, Bacterial Cellulose (BC) has numerous uses in burn treatment, and Sesamum fuel was traditionally utilized for this purpose. As a result, we used layers of Sesamum fuel-coated purified bacterial cellulose in this study. AFM and SEM were used to evaluate the specimens' bending property and wettability, and the morphology of BC was characterized. The result showed that the coated layer with sesamum fuel had 50% less bending than the untreated wet layer. Additionally, it indicates a significant decrease in layer wettability. Additionally, the treated layer's antibacterial property has been investigated. Against both gram-positive and -negative bacteria, each coated layer has demonstrated significant antibacterial properties. The results show that microbial cellulose coated with sesame fuel has a lot of potential as a modern wound dressing.

Keywords

Hydrogel, Bacterial cellulose, Morphology, Cellulose

INTRODUCTION

As a biological wound dressing for wound healing and other medical applications, bacterial cellulose has been utilized in numerous applications. This nanofibrous bacterial cellulose (BC) hydrogel can hold a lot of water and provide the necessary moisture for the wound's healing. It likewise has a reasonable similarity which decreases the responsiveness and wound torment. Organic layer keeps microorganism and poison from entering bed twisted due to nanofiber and thick design. Numerous studies have been conducted in recent years to enhance the healing properties of microbial cellulose by using materials with healing properties. Sesamum fuel was used as a good burning material to improve the healing properties of microbial cellulose in this regard, and its bending, wettability, and antimicrobial properties are evaluated (Abdurakhmonov IY et al., 2016) (Pumplin N et al., 2016).

MATERIAL AND METHOD

With the help of the Islamic Republic of Iran's Ministry of Health and Medical Education, two varieties of standard sesamum fuel were made. For the coating, seven-daypurified bacterial cellulose was used. As gram-negative and gram-positive bacteria, E. coli (AATCC 11303) and S. aureus (AATCC 6538) were utilized for antibacterial research, respectively (Koch A et al., 2014).

Purification: After purifying the bacterial cellulose layer for 90 minutes at the bfueling point with 0.1 N NaoH, samples were neutralized. Coating: Coating layers were applied using the immersion method. Dried and wet BC filtered layer is drenched for 8 hours in sesamum fuel. Steadiness in bending: Bowing firmness of microbial not set in stone by BS 3356 norm with Shirley twisting instrument. This method produces specimens with dimensions of 2.5 cm by 20 cm (Smagghe G 2019).

Wettability: The standard sinking time method (ASTM D2281-10) is used to evaluate a layer's wettability. Feature that kills bacteria: The AATCC 147 method (zone of inhibition) is used to investigate the antibacterial property of a sample treated with sesamum fuel. SEM, or scanning electron microscopy, the surface morphology of the untreated and decontaminated examples was examined by means of SEM (Model: instrument (Philips XL30, Netherlands). AFM, or Atomic Force Microscopy, AFM was used to structurally characterize the surface topology of the treated and untreated samples (Dalakouras A et al., 2015).

DISCUSSION AND RESULTS

As indicated by the significance of sodden climate around wound and furthermore no grip of twisted dressing to bed wound, sinking strategy for hydrophilic layer was applied. The hydrophobic property of a dry layer infused with sesamum fuel is higher than that of a wet layer infused with sesamum fuel. This property made no bond of wound dressing bed twisted yet decrease of water assimilation ought to be viewed as in injuries that have high exudate. Additionally, the sesamum fuel-treated four-day dried sample lacks wettability. This layer can be utilized for dry injury which has no exudate and wound's surface ought to keep fuel and damp (Bonsembiante L et al., 2021).

The outcome addressed showed that twisting solidness of treated layer by sesamum fuel is half not exactly untreated nanobacterial cellulose layer. This means that coating the layer with sesamum fuel made it more flexible, but not as much as the purified wet BC layer. Antibacterial property of two examples is assessed by zone of hindrance strategy. According to sample 1 is more antibacterial than sample 2. E. coli, a Gram-negative microbe, has more of this property than S. areous, a Gram-positive microbe. Sesamum fuel was found to not only keep the bed wound flexible and greasy, but it also stopped a bacterial infection from growing. Sesamum fuel, which has been used in medical applications for many centuries, and the inherent property of sesamum seeds are both associated with the antibacterial property (Cannata F et al., 2020).

It is evident that microbial cellulose has a three-dimensional and porous structure. Cellulosic nanofibers may be able to absorb liquid and sesamum fuel thanks to the porosity. The high specific surface of the fibres that became hydrophobic after being impregnated with fuel is linked to the layer's high hydrophobicity. The nanofibers network's porosity allows each layer to physically absorb water. The hydrophobic nature caused no attachment of nanofibers layer to the injury. Because of this property, it is possible to separate the dressing from the bed wound without causing pain or bleeding (Petersen KF et al., 2003).

In the process of wound healing, Nano-Microbial Cellulose (NMC) plays an important role. This nanofirbers hydrogel has no side effects and can hold a lot of water, relieving pain. NMC is created by biosynthesis of certain microscopic organisms which Acetobacter Xylynium is the most impressive one. This microbe can produce microbial cellulose in a variety of shapes, including pellicle and spherical, depending on the cultivation method (Al-Rasheedi AAS et al., 2014).

During the biosynthetic process, bacteria use internal metabolism to transform the sugar in the culture medium into a nanosized cellulosic fibril that is secreted into the medium through the pores on the bacteria's surface. Ribbons of fine fibrils produced nanofibers on the surface of the culture medium, immobilizing bacteria in a threedimensional network. Based on this, layer purification to get rid of microbes and other pollutant from culture mediums like pigments is a must in many applications, especially wound dressings. The raw and purified layers' permeability, wettability, and water absorption are then compared for use in medical applications (Kahn SE et al., 2006).

CONCLUSION

Various materials have been utilized in the past to enhance the healing properties. Sesamum fuel with sufficient healing properties is used in this study to improve the microbial cellulose layer's healing properties. The obtained result demonstrated that sesamum fuel can produce a microbial cellulose layer that is more hydrophobic. Due to its inherent antibacterial property, it can also stop bacterial infections from growing. Hence, microbial cellulose with sesamum fuel can possibly be utilized as a cutting edge wound dressing.

CONFLICT OF INTEREST

None

ACKNOWLEDGEMENT

None

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