Abstract
Statement of the Problem: Glycosylation is one of the
most common posttranslational modifications of proteins.
Altered glycosylation is present in many
pathophysiological conditions. Glycomic studies on rat
serum have revealed variations in the N-glycans of
glycoproteins correlated with disease progress, which is
consistent with the findings in human serum. The main
goal of our study was to describe the glycoprofiles of
different rat strains fed 5 weeks standard diet and to
evaluate their differences according to the N-glycan type.
Methodology & Theoretical Orientation: For our
observation we used Wistar rats (W), the general
multipurpose model strain. Then spontaneously
hypertensive rats (SHR) were used, developed as animal
models for human essential (idiopathic or primary)
hypertensions. Finally, hereditary hypertriglyceridemic
rats (hHTG) were also included, regarded as suitable
animal model of cardiovascular disease and metabolic
syndrome. The analysis of serum N-glycoprofilewas done
by mass spectrometry analytics on MALDI-TOF/TOF
instrumentation. Analyzed data were processed by
FlexAnalysis (Bruker Daltonics) and GlycoWork Bench
software. Findings: The cluster of 22 N-glycanswas
appointed and sorted with special impact on their
structural type. The changes in relative intensities of Nglycans were not significant, however, there were
observed some trends in its remodelation within different
rats strains. In W group there was detected higher
percentage of high-manose N-glycan type. In SHR group
was higher portion of complex-bi-antennary N-glycans
with fucose and in hHTG group higher portion of
complex-bi-antennary and complex-bi-antennary Nglycans with fucose.Conclusion & Significance: These
data of blood sera glycoprofiling in different rat strains
might assume as a possible tool for basic research to test
therapeutic perspectives within various civilization and
metabolic diseases. Further impact on clinical studies
tendencies might be considered. Acknowledgements:This
work supported by grants: EU project
ITMS2014+313021Y920, APVV-18-0336, VEGA
2/0104/21 and Ministry of Health´s SR project No.
2019/7-CHUSAV-4.
Keywords
Glycomics, Biochemistry
Glycomic studies on rat serum have revealed variations in the N-glycans of glycoproteins correlated with disease progress, which is consistent with the findings in human serum
Biochemistry is both life science and a chemical science - it explores the chemistry of living organisms and the molecular basis for the changes occurring in living cells. It uses the methods of chemistry,
"Biochemistry has become the foundation for understanding all biological processes. It has provided explanations for the causes of many diseases in humans, animals and plants."
physics, molecular biology, and immunology to study the structure and behaviour of the complex molecules found in biological material and the ways these molecules interact to form cells, tissues, and whole organisms.
Biochemists are interested, for example, in mechanisms of brain function, cellular multiplication and differentiation, communication within and between cells and organs, and the chemical bases of inheritance and disease. The biochemist seeks to determine how specific molecules such as proteins, nucleic acids, lipids, vitamins, and hormones function in such processes. Particular emphasis is placed on the regulation of chemical reactions in living cells.
