By mobilising and transporting nutrients, plant-associated microbes can promote plant growth and have an impact on crop yield and quality. Therefore, it seems that one of the important factors affecting the health and production of plants is the rhizosphere microbiome. Through the establishment of distinct chemical niches in the soil, which are mediated by the release of phytochemicals (i.e., root exudates), the roots of plants have the capacity to influence their surrounding microbiology, the rhizosphere microbiome. These factors include the genotype of the plants, the characteristics of the soil, the nutritional status of the plants, and the climatic conditions. In the current study, barley and tomatoes—two different crop species with distinctive Fe acquisition strategies—have been grown in the RHIZOtest system using either complete or Fe-free nutrient solutions to cause Fe famine. Following that, plants were grown for six days on two various calcareous soils. Total DNA was collected from the rhizosphere and bulk soil, and the V1-V3 16S rRNA gene region was sequenced using 454 pyrosequencing technology. For each sample, about 5000 sequences were retrieved(Lee, Peter et ., 2020).
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