Swapan K. Tripathy, Rajesh Ranjan, Devraj Lenka, Bhumika Ray Mohapatra and Shovina Pal
Internode explants of grass pea (Lathyrus sativus L.) pre-cultured in B5 with 2, 4-D (2mg/l) + BAP (0.5mg/l) when transferred to BM medium containing 0.5mg/l NAA + 2mg/l BAP; produced conspicuous, glossy, dark green and well developed somatic embryos. These were found to be ideal for whole plantlet differentiation in a suitable germination medium (hormone-free MS). The embryogenic cultures were maintained in BM + 0.5mg/l NAA + 2mg/l BAP for about two years with continuous induction of globular to torpedo SEs in addition to proliferation of greenish organogenic callus. Multiple shoots occasionally grew from such green calli on transfer to a shoot regenerating medium, B5 + 0.5mg/l IAA + 1mg/BAP. Dhenkanal local yielded the highest somatic embryogenic regeneration response (19.8 + 1.32%) followed by Nirmal (12.5 + 1.66%), while P24 did not respond at all. The somatic embryo- derived regenerated plantlets were readily converted into sexually mature plants.
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