+44 330 818 7254Khaled M. Essam El-Den, Mohamed A. M. El Awady, Samer, E. M. Ismai and Adel A. El-Tarras
Enzyme- linked immunosorbent assay (ELISA) testing of 25 rose (Rosa multiflora. cv Taifey) samples which exhibited virus- like symptoms of the rose mosaic disease. These samples were collected from different locations in Taif governorate, Kingdome of Saudi Arabia;. The serological assay of Double antibody sandwich indirect -enzyme linked immunosorbent (DASI-ELISA) indicated that rose mosaic disease in KSA is associated with Prunus Necrotic Ring Spot Virus ( PNRSV, Genus Ilarvirus, Family Bromoviridae and Apple mosaic virus (ApMV, Genus Ilarvirus, Family Bromoviridae either as a single or mixed infection. DASI-ELISA was used to detect PNRSV and ApMV isolates by using Polyclonal antibodies (PAbs) specific to PNRSV and ApMV. In PNRSV out of 20 tested samples, 14 samples represent 70 % gave positive reactions using ELISA with values ranged between 0.504 and 2.104 compared to values ranged between 0.065 and 0.072 of the 6 negative healthy samples. In ApMV out of 20 tested samples, 7 samples represent 35 % gave positive reactions using ELISA with values ranged between 0.064 and 0,253 compared to values ranged between 0.064 and 0.218 of the 13 negative healthy samples. The sample which exhibited mixed infection of two viruses showing rose mosaic symptoms appears as chlorotic vein banding, a mosaic pattern, and a yellow net pattern on Rosa multiflora. cv Taifey. The viral capsid protein was estimated to be 25000 Dalton of Rose Viruses Associated with Rose Mosaic Disease. This is the first report of rose viruses in Taif- KSA.
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