+44 330 818 7254A bioreactor system that can simultaneously stimulate cells with cyclic strain and ultrasound, both of which are known to effectively stimulate the regeneration of bone tissue, was developed for this study. Due to their osteoblast-like characteristics, MC3T3-E1 pre-osteoblasts were chosen for use in bone tissue engineering. PolyL-lactic acid and polycaprolactone were used in the salt leaching process to create three-dimensional scaffolds. The bioreactor used ultrasound and cyclic strain to stimulate the cells. For ultrasound, the bioreactor was set to 1.0 MHz and 30 mW/cm2 at 1.0 Hz and 10% strain for cyclic strain. A control group and three experimental groups—ultrasound, cyclic strain, and combined stimulation—were examined. For 20 minutes each day, each group was stimulated. When measured using the cell counting kit-8, mechanical stimuli had no significant effect on the proliferation of MC3T3-E1 cells for up to ten days. However, MC3T3-E1 cells' matrix maturation was accelerated by the combination of mechanical stimulation, as shown by gene expression analysis of osteocalcin, RUNX2, and osterix
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