Chen LM, Li ZT, Wang MZ, Wang HR
In this experiment, the effects of arginine levels on the synthesis and gene expressions of αs-casein and β-casein were assayed by bovine mammary epithelial cell. The treatments were designed as follows, Arg 0.25× group (69.50 mg/L), 0.5× group (139.00 mg/L), 1× group (278.00 mg/L), 2× group (556.00 mg/L), 4× group (1, 112 mg/L), 8× group (2, 224 mg/L) as experimental group respectively, and Arg 0× group (0.00 mg/L) was set as the control group. αs-casein and β-casein synthesis were tested using the ELISA kit, and the CSN1S1 and CSN2 gene expression was examined by RT-PCR method. The results showed that, compared to the control group, αs-casein synthesis in the Arg 0.5×, Arg 1× and Arg 2× group was significantly higher (P < 0.05), and highest in the Arg 2× group (P < 0.05). As for β-casein, the experimental groups were higher than control group (P < 0.05) except for the Arg 0.25× group; was also highest in the Arg 2× group (P < 0.05). It was also observed that, all experimental groups were higher than the control group in the relative expression quantities of CSN1S1 gene significantly (P < 0.05); and also higher than the control group in CSN2 gene (P < 0.05), except for the Arg 8× group. And similarly, CSN1S1 and CSN2 were all highest in the Arg 2× group (P < 0.05). In conclusion, the synthesis of αs-casein and β-casein and the expressions of CSN1S1 and CSN2 gene were all manipulated by arginine levels; and the Arg 2× group containing 556.00 mg/L arginine in medium demonstrated the highest impact.
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