Demand from consumers has led to an increase in the popularity of free-range and organic poultry in recent years. A study was done to see how organic dairy cow manure might affect the environment and the microbiology of eggs in organic free-range laying flocks. A flock of brown egg hens was divided up and kept in a rotational paddock grazing schedule that did not include or expose them to organic dairy manure. Between 20 and 44 weeks of age, environmental samples and eggs were taken in order to count the number of Enterobacteriaceae and check for the presence of Listeria, Campylobacter, and Salmonella spp. There was no discernible difference between integrated and control grazing in the prevalence of Listeria spp., Campylobacter spp., and Salmonella spp. in environmental and egg samples. 211 viable isolates from Enterobacteriaceae colonies were obtained using a random sample and biochemical characterization. There were 17 taxa, species, or serotypes found (John et al., 1997). The prevalence of total coliforms was higher (P 0.05) in integrated organic free-range flocks compared to control organic freerange flocks in the samples of shell emulsion, egg contents, nest box straw, and forage. The microbial levels retrieved from ambient and egg samples were altered by the seasons, with the summer having the greatest level of all populations under observation. To fully comprehend the impact of mixed production rotational grazing on the prevalence of pathogens and Enterobacteriaceae on organic nest-run eggs and the grazing environment, additional research is required (Goldman et al., 1996).
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