+44 330 818 7254Zhang Jing-Hu, Yang Hong-Wen and Liu Chong
The full-length cDNA sequence of one goat gene, RAP1B, was amplified using the rapid amplification of cDNA ends (RACE) method based on one goat EST sequence which was highly homologous to the coding sequence of human RAP1B gene. Sequence prediction analysis revealed that the open reading frame of this gene encodes a protein of 184 amino acids that shares high homology with the RAP1B, member of RAS oncogene family(RAP1B) of nine species—human(100%), cattle(100%), mouse(100%), dog(99%), rat(99%), umatran orangutan(99%), blue catfish(98%), giant panda(98%) and common carp(98%)—so that it can be defined as goat RAP1B gene. This novel goat gene was then deposited into NCBI database. The phylogenetic analysis revealed that the goat RAP1B gene has a closer genetic relationship with the RAP1B genes of human, cattle and mouse. Tissue expression analysis indicated that the goat RAP1B gene is generally and differentially expressed in detected tissues including spleen, muscle, skin, kidney, lung, liver, fat and heart. Our experiment established the primary foundation for further research on the goat RAP1B gene.
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