+44 330 818 7254Zhang Jing-Hu, Luo Kai-Mei, Cao Fang and Liu Chong
The full-length cDNA sequence of one goat gene, RAB7A, was amplified using the rapid amplification of cDNA ends (RACE) method based on one goat EST sequence which was highly homologous to the coding sequence of human RAB7A gene. Sequence prediction analysis revealed that the open reading frame of this gene encodes a protein of 207 amino acids that has high homology with the RAB7A, member RAS oncogene family (RAB7A) of seven species—sheep (100%), human(99%), mouse( 99%), Chinese hamster(99%), rat(99%), green anole(99%) and dog(99%)—so that it can be defined as goat RAB7A gene.. This novel goat gene was then deposited into NCBI database. The phylogenetic analysis revealed that the goat RAB7A gene has a closer genetic relationship with the RAB7A gene of sheep. Tissue expression analysis indicated that the goat RAB7A gene is differentially expressed in detected tissues including spleen, muscle, skin, kidney, lung, liver, fat and heart. Our experiment established the primary foundation for further research on the goat RAB7A gene.
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