+44 330 818 7254Sanonne, Fotso, Donfagsiteli Tchinda Nehemie, Omokolo Ndoumou Denis
African pearwood (Baillonella toxisperma Pierre) is one of the biggest trees of the Central Africa rainforest. It offers number of uses but the species is classified as vulnerable. This study is conducted in view of its domestication via somatic embryogenesis. Here we analyzed the variations of free amino acids, soluble and ionically bound proteins during different stages of somatic embryogenesis in halfstrength Murashige and Skoog and in half-strength Driver and Kuniyuki media. In both media respectively, the endogenous levels of free amino acids, soluble and ionically bound proteins respectively were low in embryogenic calli [(0.30, 0.72); (0.15, 0.72); (0.09, 0.35) mg/g FM ] then increased significantly in globular embryos [(1.60, 0.93); (0.39, 5.35); (0.17, 1.26) mg/g FM)]. Finally, the levels of all these somatic embryogenesis markers decreased significantly in bipolar embryos [(1.41, 0.54); (0.24, 1.89); (0.20, 0.84) mg/g FM] excepted in MS/2, in which ionically bound proteins content increased to 0.20 mg/g FM. Free amino acids, soluble and ionically bound proteins amounts may play a key role in globular somatic embryos formation, while bipolar somatic embryos differentiation could be associated to specific types of those biochemical markers.
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