A cyanobacterium Lyngbya bipunctata was isolated from the collected soil samples from different locations. Identification was carried out by using morphological variation and taxonomical approaches according to Desikachary (1959). The axenic culture of Lyngbya bipunctata obtained in the laboratory. For the biomass production, different culture media were used namely BG-11 medium (Rippka et al., 1979). The biomass was harvested by filtration through double layered muslin cloth and dried using air blower. Lyngbya bipunctata cultures were isolated by enriching soil samples in BG-11 medium (Kaushik 1987). Axenic cultures of Lyngbya bipunctata were obtained by repeated liquid transfer of small amounts of material followed by antibiotic treatment (streptomycin, chloramphenicol, and penicillin (10 mg ml-1) (Kaushik, 1987). Unialgal cultures were purified by successive transfer from liquid to solid media. Isolates were grown axenically in 100 ml of BG-11 medium at 7.5 pH and 24o C temperature in 300 ml glass bottles. The procedure for extraction requires solvents of varying polarity, viz, (i) hexane (ii) Chloroform (iii) Methanol and (iv) Distilled Water. The powdered biomass of Lyngbya bipunctata samples were weighed and added to each solvent in the ratio 1:10 (w/v) for metabolite extraction. Three successive extract filtrations using Whatman paper were carried out and the subsequent filtrates were collected. Treatment of HeLa cells with Methanol, chloroform, hexane and aqueous extracts resulted in dose-dependent cell killing as evidenced by the continuous reduction in cell survival evaluated by MTT assays. The extracts derived from Lyngbya bipunctata were tested and showed antineoplastic activity but with varying proportion. The most effective activity was shown by aqueous extract of Lyngbya bipunctata.
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