Iroha Ifeanyichukwu Romanus
This study was designed to determine the antimicrobial sensitivity patterns of bacterial isolates from patients admitted in Federal Teaching Hospital (FETHA) Abakaliki in the year 2011-2012. Eight- five bacterial isolates were isolated from various clinical specimen namely urine (38), wound swab (17), blood (11), sputum (8), high vaginal swab (4), stool (4), Eye swab (3) and were analyzed using standard microbiology technique in the Department of Applied Microbiology Laboratory unit of Ebonyi State University Abakaliki. Standard microbiology techniques employed were culturing of the clinical specimen onto blood agar, MacConkey and Mannitol salt agar. Organisms were identified by their colonial morphology, Gram staining and appropriate biochemical test. Antimicrobial susceptibility patterns of the bacterial isolates recovered from different clinical specimen were determined using modified Kirby and Bauer method with the following antibiotics; ampicillin, sulfametoxazole/trimethoprim, ceftazidime, ciprofloxacin, amikacin, amoxicillin/clavulanic acid, gentamicin, tobramycin, ofloxacin, clindamycin, oxacillin, erythromycin and cefotazime. Among the 289 different clinical specimens collected 85 organisms were isolated which includes; five Gram negatives (Escherichia coli (25), Klebsiella spp. (24), Proteus spp. (7) Citrobacter spp. (6) and Pseudomonas spp. (6) and two Gram-positive organisms Staphylococcus aureus (15), and Streptococcus spp. (2). Antibiotics susceptibility studies showed that Gram-negative and Gram-positive organisms were all susceptible to amikacin. Individually E. coli and Klebsiella spp. were also susceptible to ciprofloxacin, gentamicin, ofloxacin and nitrofuraintoin, Pseudomonas and Proteus spp. were susceptible to ciprofloxacin and gentamicin while Citrobacter spp. to amoxicillin/clavulanic acid. Strains of Staph. aureus and Streptococcus spp were resistant to oxacillin. Streptococcus spp. were susceptible to ciprofloxacin, ceftazidime but resistant to amoxicillin/clavulanic acid, ceftazidime and ampicillin. In conclusion resistance observed in the present study among some commonly used antibiotics pose a serious problem although high susceptibility was observed with amikacin ciprofloxacin and gentamicin etc. Therefore, we suggest the need for continuous surveillance of sensitivity patterns of antimicrobial agents in our hospital to enable us know the trend of this problem.
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