R. S. Sajeevan, S. Jeba Singh, Karaba N Nataraja, M. B. Shivanna
An efficient in vitro protocol for multiple shoot induction was standardized using nodal segment explant in a most popular mulberry, Morus alba variety V1. Explants collected from the field grown plants were cultured on Murashige and Skoog (MS) medium supplemented with different concentration/combination(s) of phytohormones. Multiple shoots were induced from the nodal segments after 45 days of incubation on shoot induction medium containing MS with BAP (1.0 mg/l), TDZ (0.1 mg/l) and NAA (0.25 mg/l). Further proliferation and elongation of adventitious buds were observed in secondary medium containing MS supplemented with BAP (1.0 mg/l), NAA (0.25 mg/l) and Gibberellic acid (GA3, 0.5 mg/l). Rooting was induced on MS medium containing indole-3-butyric acid (IBA, 0.5 mg/l) with or without charcoal (1 %, w/v) and well rooted plantlets were hardened in sterilized soilrite. In vitro grown plantlets showed 98% survival under green house conditions. The protocol developed would be of great use for mass propagation of mulberry and also support mulberry transgenic programs.
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