Moustapha Sene*1, Nowaki Hijikata2 , Ken Ushijima3 , Naoyuki Funamizu4
The objective of this study was to evaluate when and how often human urine should be applied in soil as fertilizer solution. Thus, pot test using spinach and synthetic urine was conducted in greenhouse. Seven treatments, those apply same amount of urine in different pattern and control (total 8 cases) were set to know adequate urine application way. Plant height at 35 cultivation days and fresh and dry weight of plant after harvest were significantly lower in 100% urine applied in one time before seedling (a) and control (h) than no urine applied before seedling and then every 2 days application after outbreak of plants (g). Moreover, phosphorus level content in treatment (g) (1.6±0.2 mg/g plant DW) was statistically higher than treatment (a) and control (h), both same level (0.5mg/g plant DW).The N/P ratio in (a) (95.2±24.7) was significantly different than (g) (28.4±3.5) .However, in all treatments was observed N/P >16, which means phosphorus limitation. Therefore one possible reason for the low growth in (a) might be the availability of phosphorus. Besides, leached nitrogen in (a) (38.3 %) was higher than others treatments. Hence another possible reason might be leached nitrogen. Ours results shows clearly that urine application at one time before seedling is not effective for plant growth. Furthermore urine application at frequent intervals might be a better way to promote plant growth.
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