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Full Length Research Paper
Anti-inflammatory and anti-nociceptive effects of crude
extract and fractions of Morinda morindoides
root bark
*1Olukunle J.O, 2Abatan M.O,
1Adenubi O.T, 3Oyebanji B.O and 4Ogbole
O.O
1Department
of Veterinary Physiology and Pharmacology, University of
Agriculture, PMB 2240, Abeokuta, Ogun State.
2Department
of Veterinary Physiology and Pharmacology, University of
Ibadan, Ibadan, Oyo State.
3Department
of Pharmacy, Faculty of Health Sciences, Obafemi Awolowo
University, Ile Ife, Osun State.
4Department
of Pharmacognosy, Faculty of Pharmacy, University of Ibadan,
Ibadan, Oyo State.
*Corresponding Author E-mail:
drfaks@yahoo.com; Tel:
234 810 0184 6078
Received 03 March, 2012; Accepted 22 May, 2012
This study was carried out to assess the anti-inflammatory
and anti-nociceptive effects of the extract of Morinda
morindoides root bark to confirm folkoric claims.
Anti-inflammatory and analgesic effects of the methanol
extract, solvent fractions and Chromatographic sub-Fractions
(CsF) A-F of the bioactive fractions of Morinda
morindoides root bark were determined using carrageenan
induced rat paw oedema model, thermal and acetic-acid
induced writhing method in mice. Extracts of Morinda
morindoides root bark at 400, 200 and 100mg/kg of
methanol, solvent fractions (hydro methanol, hexane,
chloroform and ethyl acetate) and bioactive CsF of the root
bark were assessed in rats, which were compared with
controls; a negative control given 10ml/kg Tween 80,
positive control groups administered with Indomethacin,
aspirin, paracetamol and morphine. The mean percentage
inhibition of paw volume was highest in rats to which
indomethacin was administered (85.65%), followed by rats
administered aspirin (68.40%) which was comparable with that
of rats dosed with 400mg/kg of Morinda morindoides
root extract (65.52%).While the chloroform fraction and
chloroform CsF A of M. morindoides at 200, and
100mg/kg respectively showed bioactivity with the highest
percentage inhibition of increase in paw volume (93%). Also,
extract increased significantly (P<0.05) the reaction time
in the hot plate test (26.0+0.7)s and the mean number of
writhing was significantly lower (P<0.05) in groups dosed
with 400mg/kg methanol extract and 100mg/kg of ethyl
acetate CsF D of Morinda morindoides root bark
extract. The study establishes the anti-inflammatory and
anti-nociceptive potential of M. morindoides root
bark extract in methanol, chloroform and ethyl-acetate
solvents.
Keywords:
Morinda morindoides,
root-bark, anti-inflammatory, anti-nociceptive.
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