Full Length Research Paper
Indomethacin during early perinatal age is associated with
low levels of anxiety and stress at pre- and post-pubertal
Vanessa Blas-Valdivia1, Edgar Cano-Europa1,
Estela Meléndez-Camargo2 and
de Neurobiología, Departamento de Fisiología.
Av. Wilfrido Massieu s/n, Esq.
Manuel L. Stampa, Col. Unidad Profesional Adolfo López
Mateos, Del. Gustavo A. Madero, C.P. 07738 Mexico, D.F
de Toxicología Hepática y Renal, Departamento de Farmacía.
Av. Wilfrido Massieu s/n, Esq. Manuel L. Stampa, Col. Unidad
Profesional Adolfo López Mateos, Del. Gustavo A. Madero,
C.P. 07738 Mexico, D.F
*Corresponding Author Email:
FAX: (525) 729-62-06
Received 12 April, 2012; Accepted 22 May, 2012
It has been observed that indomethacin has undesirable
effects on CNS like reduced white matter, cognitive
dysfunction, depersonalization, hallucination and psychosis.
So, the aim of the present work was to investigate if
neonatal administration of indomethacin causes behavioral
changes related with anxiety and stress. Thirty-two male
Sprague Dawley rats were randomly divided into four groups.
Three groups received indomethacin administration at
different postnatal day. The first group received
indomethacin at P2-P3 day; the second group was
administrated at P4-P6 day; the third group received the
administration at P7-P9 day, and the control animals (group
4) were injected with an equal volume of the vehicle. It was
analyzed the effect of indomethacin administration on the
behavioral tests (traveled distance, social contact, and
immobility response) at day 36 and 57 postnatal. It was
found that indomethacin reduced the traveled distance and
ambulatory time at pre and post-pubertal age, meanwhile
indomethacin administration at P7-P9 caused an increase of
traveled distance. Also, indomethacin increased the contact
number at pre-pubertal age with a reduction at post-pubertal
age. Finally, the indomethacin administration modified
immobility responses. We conclude that indomethacin
administration during post-natal age causes stress or
anxiety related behavior because open field test, social
interaction test, and immobility responses presented
Keywords: Indomethacin, behavioral test, anxiety,
stress, perinatal age.